Open Enzyme Research Library

Use red-teaming techniques to identify exploitable weaknesses in gout, and use creative engineering to exploit them.

Open Enzyme treats gout as an attack surface, not as a predetermined product brief. It maps causal and operational weaknesses, creates falsifiable intervention tracks, and promotes or kills them on evidence. Mission and operating principles is the canonical project definition.

Start here

• Research dashboard — current priorities, experiments, and concept index
• Cross-validation — adversarial method and track threat models
• NLRP3 exploit map — inflammatory chokepoints and intervention surface
• Gout kill-chain delivery routes — mechanism × compartment × modality
• Validation experiments — discriminating wet-lab gates
• Open questions — unresolved decisions
• Computational experiments — current COMP registry

Active track families

• Urate physiology: uricase, gut-lumen sink, ABCG2 modulators, URAT1 siRNA
• Innate immunity and resolution: NLRP3 inflammasome, complement/C5a, gasdermin-D, resolution pathways
• Engineered organisms: koji track, engineered live biotherapeutics, purine-degrading bacteria, yeast uricase
• Compounds and extracts: compounding pharmacy track, medicinal mushrooms, TCM × modern rigor, supplements
• Delivery: delivery-route matrix, blood-barrier exploits, intra-articular strategies

Koji is an active, falsifiable track—not the project. Its canonical summary is koji-track.md; detailed construct and format work remains in engineered-koji-protocol.md.

Evidence and status

This is Phase 0 research and design. Pages distinguish clinical trials, animal models, in-vitro evidence, computational results, and mechanistic extrapolation. Computational claims require current exact-artifact review. A visually distinct Research Conjecture is a grounded but untested connection: its premises carry evidence tags, while the novel leap is stated separately and paired with a discriminating observation. It is not a fifth evidence level. The site does not provide medical advice.

The live corpus represents current state. Closed actions and superseded prose are available through Git history rather than duplicated archive pages.